The most fool proof approach is to cut out and replace the stained sections.
Sealing dog urine in sub floors.
Natural dog urine cleaners white vinegar white vinegar is a good and effective natural dog urine cleaner.
Treat the rest of the sub floor with a cleaner specially formulated for pet urine.
Pull up the carpet and pad and search the subfloor for urine.
You probably don t need to replace the entire subfloor.
Kilz and zinsser b i n are two common brands that work well.
Sealant goes a long way to prevent future stains from setting in.
Cut out and replace sub floor.
After you allow the floor to dry out seal it with a subfloor sealer.
Look for products designed to block odors and stains.
Subfloors and urine don t mix.
When removing an entire room of carpet or flooring use the black light on the subfloor itself to locate pet urine stains and outline them with chalk or masking tape.
The best floor sealer for pet urine is a stain blocking primer.
Baking soda baking soda is effective in absorbing odor.
And replace the pad and carpet.
The odor you smell is from the crystallized urine in the impregnated wood.
Once the carpet is removed take a blacklight flashlight and search the floor for urine spots they will glow in dim natural light with the black light turned on.
After finishing steps 1 and 2 described above soak the area completely with undiluted white vinegar.
First remove the carpet or floor covering you can t get to the urine in the sub floor through carpet and padding or linoleum.
Step 2 apply an enzymatic pet cleaner like nature s miracle to the soiled areas and allow them to dry thoroughly for 24 to 48 hours.
In some cases removing pet urine odor from wood sub floor is impossible.
If tile just cut out the grout and replace it.
Sealing the floor you can coat the subfloor with dog and cat urine resistant paint or sealer to help protect it from future damage.
Subfloor sealing will help repel liquid not just the urine variety.
Deodorizing a subfloor that has been soaked with pet urine requires a two step approach.
When not cleaned up immediately pet urine stains and odors can seep below your flooring surface and into the subfloor.
Let it soak in well and sit for several minutes before blotting the area dry.